Evaluating the quality of responses generated by open-domain conversation systems is a challenging task. This is partly because there can be multiple appropriate responses to a given dialogue history. Reference-based metrics that rely on comparisons to a set of known correct responses often fail to account for this variety, and consequently correlate poorly with human judgment. To address this problem, researchers have investigated the possibility of assessing response quality without using a set of known correct responses. RUBER demonstrated that an automatic response evaluation model could be made using unsupervised learning for the next-utterance prediction (NUP) task. For the unsupervised learning of such model, we propose a method of manipulating a golden response to create a new negative response that is designed to be inappropriate within the context while maintaining high similarity with the original golden response. We find, from our experiments on English datasets, that using the negative samples generated by our method alongside random negative samples can increase the model’s correlation with human evaluations. The process of generating such negative samples is automated and does not rely on human annotation.