Red Blood Cell Lysis Protocol: Method II
Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water.
Warm the 1X solution to room temperature prior to use.
Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of whole blood.
Gently vortex each tube immediately after adding the lysing solution.
Incubate at room temperature, protected from light, for 10-15 minutes.
Centrifuge 350 x g for 5 minutes.
Aspirate supernatant without disturbing pellet, and resuspend the pellet in the appropriate buffer (e.g., BioLegendCell Staining Buffer, Cat. No. 420201.
