How to Make a 0.5M TCEP Stock Solution
Weigh 5.73 g of TCEP.
Add 35 ml of cold molecular biology grade water to the vial, and dissolve the TCEP.
This resulting solution is very acidic, with an approximate pH of 2.5.
Bring the solution to pH 7.0 with 10 N NaOH or 10 N KOH.
Bring the resulting solution to 40 ml with molecular biology grade water.
Aliquot into 1 ml volumes in freezer tubes and store at -20°C.
