Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250)
Suspend DNA (0.1 μg/μl) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1.1, 2.1 or CutSmart.
Add 1.0 unit of Mung Bean Nuclease per μg DNA.
Incubate at 30°C for 30 minutes.
Inactivate the enzyme by phenol/chloroform extraction or by addition of SDS to 0.01%.
Recover the DNA by ethanol precipitation.
