Marine Purity Broth
Autoclave BD Difco AC Broth and NaCl Qs to 1 L with distilled water for 20 min.
After broth has cooled, add 16 ml 2 M MgSO4 and 10 ml 1 M CaCl2.  
Dispense 2.0 or 4.5 ml per sterile tube. 
Inoculate sterile MPB with 1:5 (vol:vol) dilution of culture. 
Incubate at 22°C
