Glycerol Stock
Add 600 uL of your inoculated liquid culture to a 2 mL screw top tube.
Add 200 uL autoclaved water to the screw top tube.
Add 200 uL glycerol to the screw top tube.
Screw the top on the tube and mix by inversion several times so no layers are present in the mixture.
Place mixed screw top tube into a -80 C Freezer.
