THP1 cell line differentiation
Grow cells till confluent - cells like to grow in clumps. 
Media used - RPMI + 10% FBS + b-mercaptoethanol
Plate 50,000 c/ well in a 24 well plate in 500 ul. 
Add 10-50ng/ml of PMA for 72 hours. 
After 3 days - rest cells for 16hours. 
Gamma stimulate with 10-50U IFNg o/n - use for PAMP stimulation
