SMARTseq2 day 1
Wipe down bench with RNase zap or similar. 
Dilute oligo-dT30VN primer by adding 37µL of 100µM primer to 63µL of nuclease free H20. 
Prepare RT mix ReagentStock ConcAmt/1Amt/NFinal ConcNuclease Free H20---0.29  FS Buffer5x2 1xBetaine5M2 1MMgCl21M0.06 6mMTSO100µM0.1 1µMDTT100mM0.5 5mMSuperscript II200U/µL0.5 100URNase OUT40U/µL  10USample---------- (4.3)---------. 
Incubate at 72º for 3m, return to ice. 
Take 4.3µL of hybridized oligo-dT+RNA and put in strip tubes.
Add 5.7µL RT mix RTNum. CycleGroupTempTime1xA4290m10xB502m B422m1xC7015mholdD4hold Dilute ISPCR primers 1:100. 
Prepare PCR preamp mixComponentStockAmt/1Amt/NFinal Conc(First Strand Rxn)--10µL-------KAPA HiFi Hot Start2x12.5 1xISPCR primers1µM2.5µL 0.1µM--------------------------Final Volume 25µL.   
Add 15µL PCR preamp mix to 10µL RT. 
Preamp PCR15 cycles is the current recommendation, but there's enough that I'll try 13 Ampure Cleanup.
Use 0.6 volumes of beads, so 15µL of beads for the 25µL reaction.
Resuspend in 15.5µL EB, then measure concentrations using qubit.
