Digestion for BioBrick Assembly Kit (E0546)
Digest Upstream Part with EcoRI-HF™ and SpeI.
Digest Downstream Part with XbaI and PstI.
Digest the Destination Plasmid with EcoRI-HF™  and PstI.
Incubate all three restriction digest reactions at 37°C for 10 minutes  Heat inactivate at 80°C for 20 minutes.
