PCR Protocol for OneTaq® DNA Polymerase (M0480)
Set up the following reaction on ice:
Component25 μl reaction50 μl reactionFinal Concentration5X OneTaq Standard Reaction Buffer*5 µl10 μl1X10 mM dNTPs (#N0447)0.5 µl1 μl200 µM10 µM Forward Primer0.5 µl1 μl0.2 µM10 µM Reverse Primer0.5 µl1 μl0.2 µMOneTaq  DNA Polymerase0.125 µl0.25 µl1.25 units/50 µl PCR**Template DNAvariablevariable< 1,000 ngNuclease-free waterto 25 µlto 50 µl.  
Gently mix the reaction Collect all liquid to the bottom of the tube by a quick spin if necessary and overlay the sample with mineral oil  if using a PCR machine without a heated lid. 
Quickly transfer PCR tubes to a thermocycler preheated to the denaturation temperature (94°C) and begin thermocycling
