Nile Red Staining of Drosophila Larval Tissues
Dissect tissues in ice cold PBS, keeping samples on ice until required sample size is obtained.
Fix tissues in 4% PFA for 20 minutes.
Wash tissues 3x in PBS.
Stain samples in Nile Red at 0.5 ug/mL diluted in PBS for 1 hour.
Wash tissues 3x in PBS.
Carefully replace the PBS with mounting medium before transferring samples to slides and imaging.
Image immediately, or temporarily store slides at 4°C.
