Large (Klenow) Fragment Blunting (M0210)
DNA should be dissolved in 1x NEBuffer or T4 DNA Ligase Reaction buffer supplemented with 33 μM each dNTP.
Add 1 unit of Klenow per microgram DNA.
Incubate for 15 minutes at 25°C.
Stop reaction by adding EDTA to a final concentration of 10 mM.
Heat for 20 minutes at 75°C.
