Western Blot - Whole Embryonic Sample
Collect 100 embryos and place into eppendorf tube (1.5ml) containing 50ul of embryo wash. 
Add 3 drops of bleach and let sit for 3 mins.
Raise volume to 1.5ml using dH2O.
Spin down for 1min. 
Remove solution.
Add 1.5ml of dH2O.
Spin down 1min. 
Remove solution.
Add 1.5ml of dH2O.
Spin down 1 min. 
Remove solution with micropipette.
Add 50ul of 1X PBS. 
Add 50ul of 2X SDS SB Homogenize with pestle and 27ga needle. 
Boil for 5 mins at 98 degrees C. 
Store at -20 degrees C
