TCBS Agar Plates (500ml)
In 500ml MilliQ, add 44g TCBS Agar.
Mix thoroughly Loosen cap.
Bring to a boil in microwave to dissolve completely and boil for approximately 1 minute.
DO NOT AUTOCLAVE. 
Allow to cool to approximately 60ºC by running container until MilliQ  Under a flame, pour TCBS agar into sterile plates about half way.
Quickly run flame over surface of agar to remove any air bubbles.
