Blunting Protocol (M0203)
Dissolve DNA in any 1X NEBuffer or T4 DNA Ligase Reaction Buffer.
Supplement with 100 μM of each dNTP.
Add 1 unit of T4 DNA Polymerase per microgram DNA.
Incubate 15 minutes at 12°C.
Stop reaction by adding EDTA to a final concentration of 10 mM.
Heat for 20 minutes at 75°C (see references 1,2).
