Super simple In vivo Hoechst staining of unicellular protists
Add 1/1000 Hoechst dye (1 uL into 1 mL volume of cells).Keep cells with dye protected from light.
Incubate at room temperature for 10 minutes.Keep cells with dye protected from light.
Centrifuge cells at 1000g for 5 minutes.
Resuspend with fresh growing medium.
Use minimum volume of medium in order to plate in to the slide immediately.
Mount your slide and ready for observation under microscope.
