Transformation and Storage Solution (TSS)
ChemicalVolume/MassFinal Conc.PEG 8000 or PEG 335020 g20% (w/v)DMSO10 ml10% (w/v)MgCl2 * H2O2.03 g100 mMLB Mediumadd 100 ml (pH 6.5).
Prepare 2x TSS without DMSO and autoclave.
Add sterile DMSO after autoclaving.
Divide in 10 ml aliquots and store at 4 °C.
