PNGase F Protocol, Denaturing Conditions
Combine the following (for a 10 µl total reaction volume): 
Denature glycoprotein by heating reaction at 95°C for 5 minutes.
Chill denatured glycoprotein on ice. 
Centrifuge 10 seconds. 
Add the following (bringing total reaction volume to 20 µl):  
Add PNGase F,  1 µl. 
Mix gently. 
Incubate reaction at 37°C for 1 hour.  
Analyze by method of choice
