BL21 Electro­competent Prep
Dilute 5mL O/N into 500mL LB.
Grow to OD 0.6­-0.8 (~1.5­-3 hours).
Pour into 2x250mL Centrifuge bottles.
Chill bottles on ice for 10 minutes.
Spin at 3000xg for 20min at 4C.
Decant supernatant.
Resuspend + combine pellets in 250mL pre­chilled 10% glycerol.
Spin at 3000xg for 20min at 4C.
Decant supernatant.
Resuspend pellet in 20mL pre­chilled 10% glycerol (switch to 50mL Falcon Tube).
Spin at 3000xg for 20min at 4C.
Decant supernatant.
Resuspend in 3mL pre­chilled 10% glycerol.
SNAP Freeze in 25­50uL aliquots in LN2.
