RNAlater Recipe
Prepare 0.5 M EDTA.
Prepare 1 M Sodium citrate.
In a 2 or 3 L flask mix 1,400 mL MilliQ water with 60 mL 0.5M EDTA and 37.5 mL 1M sodium citrate with stirring.
While continuing to stir the solution, add 1.05 kg (= 1,050 g) Ammonium sulfate in 100 g amounts.
Stir ~30 min on hot plate set to low.
Allow solution to cool at room temperature for at least 30min, but overnight on bench is best.
Filter solution through 0.2 μm filter to remove particles.
Store at room temp or at 4°C.
