First Strand Synthesis with Reverse Transcriptase
In a sterile microfuge tube add the following: 
Heat for 3-5 minutes at 65-80°C.
Spin briefly and place promptly on ice.
Add 10X RT Buffer. 
Add RNAse inhibitor 1 µL.  
Add M-MuLV Reverse Transcriptase 1 µL.  
Incubate at 42°C for one hour.
Inactivate enzyme at 90°C for 10 minutes.
Store products at -20°C or proceed to next step(s).
