Ethanol precipitation of nucleic acids ( Eppendorf tubes )
Add 1 / 10 volume of 3M sodium acetate , pH 5.2 or 1 / 2 volume of 5M ammonium acetate .
Add 2 - 3 volumes of 100 % Ethanol .
Mix and freeze overnight in - 20 .
Spin at full speed in a standard microcentrifuge at 4 degrees for 30 minutes .
Decant ( or carefully pipet off ) the supernatant .
Dry the pellet .
Add your desired quantity of water .
Vortex and spin down to resuspend .
