ESAW Media for Marine Phytoplankton
Na2SO4 NaCl KCL NaHCO3 KBr H3BO NaF ( 2.7 g / L stock ) .
Bring to 500 mL MilliQ water MgCl2 - 6H2O CaCl2 - 2H2O SrCl2 - 6H2O Tris - HCL ( pH 7.8 ) ( 1.0 M stock ) Fe - EDTA K2HPO4 ( 10g / L stock ) NaNO3 ( 550 mM stock ) Selenite ( 10 μM stock ) .
Bring to 500 mL MilliQ water .
Autoclave Solution I and Solution II separately .
Combine Solution I and Solution II .
Add trace Metals Solutions .
Add Vitamin Solution .
