Swift™ Membrane Stain
Following protein transfer to a membrane , place the membrane in a suitable tray .
a . For PVDF Membranes :
Add 20ml 100 % methanol and rinse for 10‐30 seconds .
Discard the methanol and immediately add 12ml Swift™ Membrane Stain .
b . For Nitrocellulose Membranes :
Add 20ml deionized water and rinse for 30 seconds .
Discard the wash and add 12ml Swift™ Membrane Stain .
Place the trays on a rocking shaker .
Protein bands appear in ~ 30 seconds .
Rinse the membrane in DI water to remove the staining solution for improved image capture by scanning or photography .
For rapid de‐staining of the membranes , rinse the membrane in DI water to remove staining solution .
Wash the membranes for 30‐60 seconds in 50ml 1X Swift™ Destain , or until the stain has been removed .
Once de‐stained , rinse in DI water and store the blot in DI water until required .
