YES Rich Medium
Add 5.0 g Yeast Extract to a 1 Liter Erlenmeyer Flask .
Add 30 g Glucose or Dextrose to the flask .
Add 0.25 g Adenine to the flask .
Add 0.25 g Uracil to the flask .
Add 0.25 g Histidine to the flask .
Add 0.25 Leucine to the flask .
Add 0.25 g Lysine to the flask .
Add 1 g Arginine to the flask .
Optional : For solid medium add 20 g Difco Bacto Agar to the flask .
Fill the flask with water up to the 1 Liter mark on the flask .
Place a magnetic stir bar into the flask and place the flask on a magnetic stirrer to dissolve the powder into the water .
Remove the stir bar with a magnetic rod .
Place aluminum foil over the top of the flask and then autoclave on a liquid cycle for 20 minutes .
If solid medium , pour while still hot into plates and let them sit out to cool overnight on the lab bench . If liquid medium , aliquot into smaller amounts and store at + 4 C .
