Sampling for extracellular phage quantification by qPCR and MPN assays
Place filter plate ( MSGVN2210 ) onto receiver 96 - well plate without touching the bottom of the sterile filter plate .
Keep a log of your sample layout ( which samples are in which wells ) .
Plate - Setup Template : Transfer 250 µL of sample from each experimental bottle / tube into empty wells on the filter plate in duplicate .
It is not necessary to fill the entire plate ; we will continue to use the same plate over the course of the experiment .
Centrifuge at 1000 x g for 3 min .
Be sure to balance the centrifuge .
Between sampling , store stacked filter / filtrate plates at 4°C , covered with a plate lid .
Do not disturb the stacked plates , to avoid cross - contamination between wells .
After filling the entire plate , remove the filter plate and discard .
Store the filtrate plate at 4°C , covered with a plate lid and wrapped well with parafilm , until analysis .
