Buccopharyngeal morphology of tadpoles in Scanning Electron Microscope
Wash quickly the specimens in tap water and dissect tadpoles following Wassersug ( 1976 : p . 4 - 5 ) .
Prepare the fixing solution .
Fix the dissections in 4 % Glutaraldehyde diluted in the fixing solution .
Post fix the dissections in 1 % Osmium tetroxide solution .
Dehydrate in an ascending series of acetones , starting at 30 % .
Transfer to acetone 50 % .
Transfer to acetone 70 % .
Transfer to acetone 90 % .
Transfer to acetone 95 % .
Transfer to another solution of acetone 95 % .
Transfer to another solution of acetone 95 % .
Transfer to acetone 100 % .
Transfer to another solution of acetone 100 % .
Now take your specimens to a Critical Point Dryer .
Mount the specimens on stubs and attach them with a double - sided tape .
Take the specimens to the High Vaccum Coater and you're ready to analyze them .
