Investigating non - specific protein binding and streptavidin binding using QCM - D sensing
Prepare a Hepes Buffered Saline ( HBS ) solution containing 150 mM NaCl and 10 mM Hepes .
Then prepare a solution of 25 µg / ml streptavidin in HBS .
Using QCM - D sensing , make a baseline of the HBS solution .
Expose the modified sensors to non - diluted Fetal Bovine Serum ( FBS ) for 30 - 60 min under static conditions in QCM - D and measure the amount of adsorbed FBS relative to the HBS baseline obtained in the previous step .
Rinse the sensors with the HBS solution .
Flow the streptavidin solution over the modified sensors ( 100 - 500 µl / min ) , until they are saturated .
Rinse the sensors with the HBS solution .
