Release of nucleic acids with heat , chelator , and detergent
Concentrate the viruses by centrifuging in the centrifugal ultrafiltration device at 1000g until only a small volume ( ca . 10 µL ) remains .
Add 100 µL TE ( or TEGED ) .
Concentrate the sample again to ca . 10 µL .
Repeat steps 2 and 3 once more .
Recover the final concentrate .
Rinse the membrane in the device by adding a small volume of TE or TEGED ( 5–10 µL ) .
Recover the rinse and pool with the concentrate .
Optional : If conducting electrophoresis on the sample , add SDS - EDTA loading dye to a final concentration of 1× .
Heat the recovered sample ( with or without loading buffer ) to 60°C for 10 min to release the nucleic acid .
