Dephosphorylation using rSAP in Restriction Enzyme Reaction ( M0371 )
Digest 1–5 μg of plasmid DNA in a 20 μl reaction as follows : Incubate at 37°C for 60 minutes or follow manufacturer’s recommendations .
Add 1 unit of rSAP for every 1 pmol of DNA ends ( about 1 μg of a 3 kb plasmid ) .
Incubate at 37°C for 30–60 minutes .
Stop reaction by heat - inactivation of rSAP and restriction enzyme ( follow manufacturer's recommendations ) .
