Protein Coomassie Blue Staining
Submerge protein acrylamide gel in coomassie blue stain and shake at room temperature for two hours .
Pour off stain into a bottle ( you can reuse it a few times ) , wash gel with water , then pour more stain on and shake at room temperature for another two hours .
Pour off stain into a bottle , wash gel with water , you should be able to see a little something at this point so take a picture , then pour more stain on and shake at room temperature overnight .
Pour off stain and take a picture of the protein bands .
Submerge gel in destain buffer ( 50 mL Acetic Acid , 100 mL Methanol , 350 mL Water ) for an hour and shake at room temperature .
Wash with water then repeat step 5 until you can clearly see the protein bands distinct from the background of the gel .
