Digestion for BioBrick Assembly Kit ( E0546 )
Digest Upstream Part with EcoRI - HF™ and SpeI .
Digest Downstream Part with XbaI and PstI .
Digest the Destination Plasmid with EcoRI - HF™ and PstI .
Incubate all three restriction digest reactions at 37°C for 10 minutes Heat inactivate at 80°C for 20 minutes .
