Concentrating Viruses with an Amicon or Nanosep Centrifugal Ultrafiltration Device
Add your sample to the upper reservoir of the Amicon .
Centrifuge at 1000 x g at 4ºC in a swinging - bucket rotor .
Centrifuge time will depend on the volume of sample and the amount of material in the sample .
Continue centrifuging until the sample is reduced to the desired volume .
Remove the concentrated sample from the upper reservoir and place it in a tube of your choice .
Remove the upper reservoir from the Amicon device .
Place a square of parafilm over the bottom of the upper reservoir ( Figure 1 ) .
Add enough liquid to cover the filter ( ~ 1.5 ml ) .
Vortex the upper reservoir ( filter / parafilm side down ) at a setting of 1200 for ~ 20s .
Remove the liquid from the upper reservoir and add it to your recovered sample .
Repeat steps 8 - 10 to fully recover your sample .
Add your sample to the upper reservoir of the Nanosep .
Centrifuge at 1000 x g at 4ºC .
Centrifuge time will depend on the volume of sample and the amount of material in the sample .
Continue centrifuging until the sample is reduced to the desired volume .
Use a pipettor to remove the concentrated sample from the upper reservoir and place it in a tube of your choice .
Add enough liquid to cover the filter ( ~ 10 µl ) .
Swirl the liquid over the filter and stir it gently with a pipet tip .
Remove the liquid from the upper reservoir and add it to your recovered sample .
Repeat steps 17 to 19 to fully recover your sample .
