Quick Ligation Protocol ( M2200 )
Combine 50 ng of vector with a 3 - fold molar excess of insert .
Adjust volume to 10μl with dH2O .
Add 10 μl of 2X Quick Ligation Buffer and mix .
Add 1μl of Quick T4 DNA Ligase and mix thoroughly .
Centrifuge briefly and incubate at room temperature ( 25°C ) for 5 minutes .
Chill on ice , then transform or store at - 20°C .
