Modified KAPA Express Extract
Incubate in a thermocycler for 10 minutes at 75ºC .
During this step , cells are lysed , nucleases and proteins degraded and DNA released .
Incubate for 5 minutes at 95ºC to inactivate the thermostable KAPA Express Extract protease .
Vortex reaction product for 2 - 3 sec .
Centrifuge at high speed for 1 minute to pellet debris . Transfer DNA - containing supernatant to a fresh tube .
Use 1 μl of DNA extract directly in a 25 μl PCR , without quantification .
Dilute in TE Buffer for long - term storage at - 20ºC .
