MojoSort™ Positive Selection Columns Protocol
Prepare a single cell suspension and resuspend the cells with ice cold cell separation buffer ( MojoSort™buffer is recommended ) .
Pass the cells through a 70 μm filter , centrifuge ( 300 x g for 5 minutes ) , discard the supernatant and resuspend the cells in cell separation buffer .
Adjust the cell concentration to 1 x 108 cells / mL .
Aliquot 100 μL ( 107 cells ) into a new tube .
Vortex the antibody - conjugated Nanobeads ( to resuspend ) at max speed , 5 touches , and prepare the dilutions to test .
Add 10 μL of pre - diluted conjugated Nanobeads .
Mix well and incubate on ice for 15 minutes .
Scale up the volume accordingly if separating more cells .
For example , add 100 μL of pre - diluted Nanobeads for 1 x 108 cells .
When working with less than 107 cells , use indicated volumes for 107 cells .
Note : Depending on the conjugated nanobead you are using , a wash step may be required here .
Resuspend the cells in appropriate amount of buffer .
At least 500 μL is needed for column separation .
Note : There are several types of commercially available columns , depending on your application , choose the one that fits best your experiment .
See 'Guidelines' for choosing a column for your experiment .
