IPTG – 1 M ( 100 x ) Stock Solution
Weigh 2.383 g of IPTG .
Add 10 ml sterile H2O .
Dissolve completely .
Prewet a 0.2 µm syringe filter by drawing through 5 - 10 ml of sterile H2O and discard water .
Sterilize Ampicillin Stock through the prepared 0.2 µm syringe filter .
Store in 1 ml aliquots at - 20°C for up to 1 year .
