Large ( Klenow ) Fragment Blunting ( M0210 )
DNA should be dissolved in 1x NEBuffer or T4 DNA Ligase Reaction buffer supplemented with 33 μM each dNTP .
Add 1 unit of Klenow per microgram DNA .
Incubate for 15 minutes at 25°C .
Stop reaction by adding EDTA to a final concentration of 10 mM .
Heat for 20 minutes at 75°C .
