Electron Microscopy of Haloviruses
A sample of virus is placed on a sterile surface and the grid , plastic - coated side down , placed on the droplet for 1.5–2 min .
The grid is then placed , for 1–1.5 min , on a drop of freshly filtered 2 % w / v uranyl acetate and excess stain removed with filter paper .
After air drying , grids may be examined by transmission electron microscopy , as described in guidelines ( Fig . 3A , B , and C in guidelines ) .
