Gel DNA Recovery
Excise up to 200 mg gel slice containing the DNA fragment using a clean scalpel or razor blade .
Cut as close to the DNA as possible to minimize the gel volume .
Place the gel slice into a 1.5 mL tube .
Add 200 µL of Extraction Buffer .
Mix thoroughly by pippeting .
Incubate the gel mixture at 50 - 58°C for 10 minutes or until the gel slice is completely dissolved .
Mix the tube by inversion every few minutes to facilitate the melting process .
Ensure that the gel is completely dissolved .
Add 200 µL of ethanol ( 96 - 100 % ) and mix by pipetting .
Transfer the mixture to the DNA Purification Micro Column preassembled with a collection tube .
Centrifuge the column for 1 minute at 14,000 × g .
Discard the flow - through .
Place the DNA Purification Micro Column back into the collection tube .
Add 200 µL of Prewash Buffer ( supplemented with ethanol , see p . 3 ) to the DNA Purification Micro Column and centrifuge for 1 minute at 14,000 × g .
Discard the flow - through and place the purification column back into the collection tube .
Add 700 µL of Wash Buffer ( supplemented with ethanol , see p . 3 ) to the DNA Purification Micro Column and centrifuge for 1 minute at 14,000 × g .
Discard the flow - through and place the purification column back into the collection tube .
Repeat step 7 Centrifuge the empty DNA Purification Micro Column for an additional 1 minute at 14,000 × g to completely remove residual Wash Buffer .
Transfer the DNA Purification Micro Column into a clean 1.8 mL microcentrifugetube Add 10 µL of Elution Buffer to the DNA Purification Micro Column .
Centrifuge for 1 minute at 14,000 × g to elute DNA .
