SpinOUT™ : Buffer Exchange
Mark one side of the column and ensure in all centrifugations the mark is facing outwards during centrifugation .
Prepare the SpinOUT™ column by centrifuging the SpinOUT™ columns at 1,000g for 1 minute to compact the resin .
Remove the top and then bottom caps .
Place into an appropriate collection tube .
Centrifuge the column at 1,000g for 2 minutes to remove the storage buffer .
Place the column in a new collection tube and remove the cap .
Add the buffer to be exchanged into to the columna .
For 0.1ml column , use 75µl bufferb .
For 1ml column , use 0.5ml bufferc .
For 3ml column , use 1.5ml bufferd .
For 5ml column , use 2.5ml buffere .
For 10ml column , use 5ml buffer Centrifuge the column at 1,000g for 2 minutes to remove the buffer .
Repeat steps 6 and 7 , a further five more times , ensuring the buffer is discarded after each centrifugation .
Place the column in a new collection tube and remove the cap .
Slowly , apply the protein solution to the center of the SpinOUT™ resin .
The recommended volumes to apply to the column are as follows
Discard the column .
