Gel Electrophoresis
Pour 50 mL of 1X TAE Buffer into an Erlenmeyer Flask .
Weigh out 0.5 g Agarose and add it to the Erlenmeyer Flask .
Place Erlenmeyer Flask in a microwave on high power for two minutes or until solution is clear and agarose is completely dissolved .
Remove Erlenmeyer Flask from microwave and let it sit on the lab bench to cool just until you can comfortably pick it up .
Add 5 uL Ethidium into the flask and swirl to mix , taking care not to introduce bubbles .
Place gel tray on clamp and clamp securely .
Add well plates where you want wells and use a level to ensure it is balanced .
Pour contents of the Erlemeyer Flask into the gel tray and let it sit for 30 minutes , or until a blue tint appears .
Remove the well plates carefully as to not tear the gel and remove the tray from the clamp , but ensure the gel remains in the tray .
Place gel tray into gel electrophoresis apparatus with the wells closer to the negative / black end .
Pour additional TAE Buffer to fill each side of the apparatus and to create a thin layer of buffer covering the top of the gel .
Pipette 10 uL of the 1kb DNA Ladder with Loading Dye into a well .
Typically this is placed into one of the wells near an edge .
Pipette your DNA with Loading Dye mixture into another well .
Repeat for each sample .
Place lid on apparatus and plug cables into amplifier .
Set amplifier to stay at a constant voltage of 100 V . Let run for 30 minutes or until the loading dye has sufficiently moved .
Remove gel from gel tray after draining excess TAE Buffer and place on plastic wrap .
Place gel with plastic wrap on UV lamp to view bands , or store in the plastic wrap at + 4 C for later use .
