The recent development of Multimodal Large Language Models (MLLMs) has significantly advanced AI’s ability to understand visual modalities. However, existing evaluation benchmarks remain limited to single-turn question answering, overlooking the complexity of multi-turn dialogues in real-world scenarios. To bridge this gap, we introduce MT-Video-Bench, a holistic video understanding benchmark for evaluating MLLMs in multi-turn dialogues. Specifically, our MT-Video-Bench mainly assesses six core competencies that focus on perceptivity and interactivity, encompassing 1,000 meticulously curated multi-turn dialogues from diverse domains. These capabilities are rigorously aligned with real-world applications, such as interactive sports analysis and multi-turn video-based intelligent tutoring. With MT-Video-Bench, we extensively evaluate various state-of-the-art open-source and closed-source MLLMs, revealing their significant performance discrepancies and limitations in handling multi-turn video dialogues. The benchmark will be publicly available to foster future research.