There has been significant prior work using templates to study bias against demographic attributes in MLMs. However, these have limitations: they overlook random variability of templates and target concepts analyzed, assume equality amongst templates, and overlook bias quantification. Addressing these, we propose a systematic statistical approach to assess bias in MLMs, using mixed models to account for random effects, pseudo-perplexity weights for sentences derived from templates and quantify bias using statistical effect sizes. Replicating prior studies, we match on bias scores in magnitude and direction with small to medium effect sizes.Next, we explore the novel problem of gender bias in the context of *personality* and *character* traits, across seven MLMs (base and large). We find that MLMs vary; ALBERT is unbiased for binary gender but the most biased for non-binary *neo*, while RoBERTa-large is the most biased for binary gender but shows small to no bias for *neo*. There is some alignment of MLM bias and findings in psychology (human perspective) - in *agreeableness* with RoBERTa-large and *emotional stability* with BERT-large. There is general agreement for the remaining 3 personality dimensions: both sides observe at most small differences across gender. For character traits, human studies on gender bias are limited thus comparisons are not feasible.

While affective expressions on social media have been extensively studied, most research has focused on the Western context. This paper explores cultural differences in affective expressions by comparing valence and arousal on Twitter/X (geolocated to the US) and Sina Weibo (in Mainland China). Using the NRC-VAD lexicon to measure valence and arousal, we identify distinct patterns of emotional expression across both platforms. Our analysis reveals a functional representation between valence and arousal, showing a negative offset in contrast to traditional lab-based findings which suggest a positive offset. Furthermore, we uncover significant cross-cultural differences in arousal, with US users displaying higher emotional intensity than Chinese users, regardless of the valence of the content. Finally, we conduct a comprehensive language analysis correlating n-grams and LDA topics with affective dimensions to deepen our understanding of how language and culture shape emotional expression. These findings contribute to a more nuanced understanding of affective communication across cultural and linguistic contexts on social media.