Massively multilingual pre-trained language models (MMPLMs) are developed in recent years demonstrating superpowers and the pre-knowledge they acquire for downstream tasks.This work investigates whether MMPLMs can be applied to clinical domain machine translation (MT) towards entirely unseen languages via transfer learning.We carry out an experimental investigation using Meta-AI’s MMPLMs “wmt21-dense-24-wide-en-X and X-en (WMT21fb)” which were pre-trained on 7 language pairs and 14 translation directions including English to Czech, German, Hausa, Icelandic, Japanese, Russian, and Chinese, and the opposite direction.We fine-tune these MMPLMs towards English-Spanish language pair which did not exist at all in their original pre-trained corpora both implicitly and explicitly.We prepare carefully aligned clinical domain data for this fine-tuning, which is different from their original mixed domain knowledge.Our experimental result shows that the fine-tuning is very successful using just 250k well-aligned in-domain EN-ES segments for three sub-task translation testings: clinical cases, clinical terms, and ontology concepts. It achieves very close evaluation scores to another MMPLM NLLB from Meta-AI, which included Spanish as a high-resource setting in the pre-training.To the best of our knowledge, this is the first work on using MMPLMs towards clinical domain transfer-learning NMT successfully for totally unseen languages during pre-training.