First Strand Synthesis with Reverse Transcriptase
In a sterile microfuge tube add the following :
Heat for 3 - 5 minutes at 65 - 80°C .
Spin briefly and place promptly on ice .
Add 10X RT Buffer .
Add RNAse inhibitor 1 µL .
Add M - MuLV Reverse Transcriptase 1 µL .
Incubate at 42°C for one hour .
Inactivate enzyme at 90°C for 10 minutes .
Store products at - 20°C or proceed to next step ( s ) .
