NATURAL SEAWATER - BASED PRO99 MEDIUM
Prepare a glass filter funnel and flask by cleaning with acid and Milli - Q water .
Filter raw seawater through 47mm Whatman GF / F stacked on top of a 47mm 0.2µm polycarbonate filter .
Autoclave seawater in a Teflon bottle ( 60 minutes for 2L ) .
Allow to cool overnight .
Prepare 0.5M NH4Cl solution .
Prepare 0.025M NaH2PO4·H2O solution .
Prepare primary trace metal stocks .
Prepare trace metal working stock .
To make up the media , add following volumes of sterile nutrients and trace metal stock to one liter of the autoclaved seawater .
NutrientVolumeFiltered Seawater 1000.0mL0.5M NH4Cl 1.6mL0.025M NaH2PO4·H2O 2.0mLTrace metal working stock 100µL .
Store at room temperature for up to one month .
