TCBS Agar Plates ( 500ml )
In 500ml MilliQ , add 44g TCBS Agar .
Mix thoroughly Loosen cap .
Bring to a boil in microwave to dissolve completely and boil for approximately 1 minute .
DO NOT AUTOCLAVE Allow to cool to approximately 60ºC by running container until MilliQ .
Under a flame , pour TCBS agar into sterile plates about half way .
Quickly run flame over surface of agar to remove any air bubbles .
