Transformation and Storage Solution ( TSS )
ChemicalVolume / MassFinal Conc . PEG 8000 or PEG 335020 g20 % ( w / v ) DMSO10 ml10 % ( w / v ) MgCl2 * H2O2.03 g100 mMLB Mediumadd 100 ml ( pH 6.5 ) .
Prepare 2x TSS without DMSO and autoclave .
Add sterile DMSO after autoclaving .
Divide in 10 ml aliquots and store at 4 °C .
