Capped RNA Synthesis ( E2050 )
Prepare 40 mM cap analog .
Thaw the necessary kit components , mix and pulse - spin in a microfuge to collect solutions to the bottoms of tubes .
Assemble the reaction at room temperature in the following order .
Mix thoroughly and pulse - spin .
Incubate at 37°C for 2 hours .
Optional step : To remove template DNA , add 2 μl of DNase I ( RNase - free ) , mix and incubate at 37°C for 15 minutes .
Proceed with purification of synthesized RNA or analysis of transcription products by gel electrophoresis
