HiFi DNA Assembly ( NEB )
Incubate samples in a thermocycler at 50°C for 15 minutes ( when 2 or 3 fragments are being assembled ) or 60 minutes ( when 4–6 fragments are being assembled ) .
Following incubation , store samples on ice or at –20°C for subsequent transformation .
Transform NEB 5 - alpha Competent E . coli cells ( provided in the cloning kit or purchased separately from NEB ) with 2 μl of the assembled product , following the transformation protocol .
