Lowry Protein Assay
Set up a standard curve of 0 , 10 , 25 , 50 , 100 , 150 and 200 µg of BSA in 10 - 15 ml tubes , 1.0 mL volume per sample .
Set up tubes ( 10 - 15 mL ) of the unknown protein sample ( s ) to determine their concentration , the final volume per sample is to be 1.0 mL .
Add 5.0 mL of reagent “C” to each tube , mix immediately .
Let the samples sit for 10 min at room temperature .
Add 0.5 mL of reagent “D” to each tube , mix immediately .
Let the samples sit for 30 min at room temperature .
Read absorbance at 600 nm .
Record values , plot standard curve and determine sample concentration ( s ) .
