Removal of Single - Stranded Extension using Mung Bean Nuclease ( M0250 )
Suspend DNA ( 0.1 μg / μl ) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1.1 , 2.1 or CutSmart .
Add 1.0 unit of Mung Bean Nuclease per μg DNA .
Incubate at 30°C for 30 minutes .
Inactivate the enzyme by phenol / chloroform extraction or by addition of SDS to 0.01 % .
Recover the DNA by ethanol precipitation .
