Phenol extraction of Chlorella cells
Pellet cells ( ~ 1 / 2 daily culture ) .
Suspend in 15 ml of liquid phenol .
Add about 0.2ml 5 % cetylpyridium chloride ( cationic detergent ) to facilitate chlorophyll extraction .
Shake suspension until green has been extracted from cells .
Add 10 ml toluene ( to reduce density ) .
Mix .
Centrifuge and wash pellet with aqueous buffer .
Wash pellet about 5x .
Use your nose as a guide to removal of phenol .
If need be a UV spectrum of the final wash supernatant can be used to assure absence of phenol .
