SYBR Green or Gold Staining
Make dilution of virus prep in 0.02µm filtered seawater ( 100 kDa permeate if available ) .
Add diluted virus to 1 ml 0.02 µm filtered seawater and add 1 µl SYBR green or gold ( 10,000x stock ) to solution .
Incubate RT , in the dark for 15 min .
Prepare 25mm SYBR towers : use 0.02µm Anodiscs , careful not to break membrane .
Filter sample onto membrane ( use vacuum ) .
Rinse tower in 1L Q - water in between samples and blot onto paper towel to dry .
Remove membrane and place cell - side up on microscope slide .
Pipet 32µl 0.1 % phenylenediamine ( made in PBS / 50 % glycerol ) on 22mm square coverslip .
Invert over filter on microscope slide and press down to spread mounting medium .
Place slide at –20°C to enhance fluorescence .
Read slides using 100x oil immersion objective and inverted fluorescent microscope .
