THP1 cell line differentiation
Grow cells till confluent - cells like to grow in clumps .
Media used - RPMI + 10 % FBS + b - mercaptoethanol
Plate 50,000 c / well in a 24 well plate in 500 ul .
Add 10 - 50ng / ml of PMA for 72 hours .
After 3 days - rest cells for 16hours .
Gamma stimulate with 10 - 50U IFNg o / n - use for PAMP stimulation
