General tube bacteria extraction ( DNeasy )
Place Buffer ATL and Buffer Al at 56C for at least 5 minutes before starting .
Make sure AW1 and AW2 have ethanol added .
Spin > 500ul of bacteria liquid culture at 10000g for 10 minutes .
Pour off supernatant .
Add 200ul buffer ATL to each sample and vortex for 15s .
Add 200ul 100 % ethanol to each sample and vortex for 15s .
Briefly spin to collect all liquid .
Transfer all of sample including precipitate to spin column .
Centrifuge at max speed ( > 20000g ) for 2 minutes .
Empty collection tube .
Add 500ul buffer AW1 to spin column .
Spin at max speed ( > 20000g ) for 2 minutes .
Add 500ul buffer AW2 to spin column .
Spin at max speed ( > 20000g ) for 2 minutes .
Place spin column in a new collection tube and spin at max speed ( > 20000g ) for 3 minutes .
Transfer spin column to a labeled 1.5ml Eppendorf lobind tube .
Add 100ul of buffer TE directly to membrane / filter in spin column and incubate at room temp .
for 1 min Spin at 10000g for 2 minutes
