Filtering for Cell Collection Pump → Steel filter tank → A / E filter → Durapore → DI rinsed cubie container ( the one the sample was in ) .
Clamp all tubes to manifolds and filter tanks .
Make sure bleeder valves are on top .
Turn on pump and then bleed A / E then Durapore and the A / E again .
Keep cubie container on ice - water will be used for viral concentration .
Place “out” tube from Durapore onto a filter flask that is attached to the vacuum side of the pump .
Wait until water stops moving towards the vacuum and only bubbles are coming through .
Open steel filter tank lid and slowly open tank bleeder valve to depressurize ( SLOWLY ) .
When pressure has dropped significantly push in steel tank lid , allow a few more minutes for water to drain through .
Disconnect clamp from bottom side of A / E filter , remove tube from “out” side of A / E .
Place Durapore on knees and swirl gently so all water goes into the filter flask .
Once water is out , open manifold slowly , fold up filter and place in whirlpack .
Remove Durapore “out” tube from filter flask , place A / E “out” tube onto filter flask .
Place A / E on knees , let water drain out into the filter flask as before .
Open manifold slowly , peel off ring of filter , discard , fold filter and place in whirlpack .
Okay to turn off pump now .
