Ethanol precipitation of nucleic acids (Eppendorf tubes)
Add 1/10 volume of 3M sodium acetate, pH 5.2 or 1/2 volume of 5M ammonium acetate.
Add 2-3 volumes of 100% Ethanol.
Mix and freeze overnight in -20.
Spin at full speed in a standard microcentrifuge at 4 degrees for 30 minutes.
Decant (or carefully pipet off) the supernatant.
Dry the pellet.
Add your desired quantity of water.
Vortex and spin down to resuspend.
