MBBM Media
Prepare 10 stock solutions per 1 liter Milli-Q H2O each: 25.0 g, NaNO3; 2.5 g, CaCl2*2H2O; 7.5 g, MgSO4*7H2O; 7.5 g, K2HPO4; 17.5 g, KH2PO4; 2.5 g, NaCl; 50.0 g, disodium EDTA; 31.0 g, KOH; 4.98 g, FeSO4*7H2O; per 1 liter acidified H2O (acificied H2O: 999.0 mL, Milli-Q H2O; and 1.0 mL, concentrated H2SO4) 11.42 g, H3BO3; 8.82 g, ZnSO4*7H2O; 1.44 g, MnCl2*4H2O; 0.71 g, MoO3; 1.57 g, CuSO4*5H2O; and 0.49 g, CoNO3*6H2O.  
Add to 950 mL, Milli-Q H2O: 1.0 g, bacto-peptone; 5.0 g, sucrose; 10.0 mL of stock solution 1-6; 1.0 mL of stock solutions 7-9; 2.0 mL stock solution 10.  
For MBBM agar plates, add 1.5% agar prior to autoclaving. 
For MBBM soft agar, add 0.75% agar prior to autoclaving. 
Autoclave at 121°C for 20 min. 
Let media cool to room temperature and add antibiotics:* 700 µg/mL, Ampicillin; and/or* 10 µg/mL, Tetracycline.
