Plaque Assay Protocol for Pseudoalteromonas
First , autoclave the top agar to melt it .
While the autoclave is running :
a . Let your plates warm to room temperature and label them
b . Make whatever dilutions of your phages you plan to plate .
Aliquot the agar into tubes and place in 40°C water bath .
Pipet 100 μl of your phage sample onto the plate .
Remove the agar tube from the water bath and add the host to it .
Add 3.5 ml of the agar / host mixture .
Swirl each plate to spread the agar as you go .
Leave the plates on the bench overnight .
Count or pick plaques the next day .
