DNA Precipitation Protocol
Add 2 volumes of ice cold ethanol , mix well .
Let sit on ice 15 - 30 minutes .
Microfuge at max speed , 20 minutes .
Carefully remove supernatant and fill tube halfway with 70 % ethanol .
Spin at max speed for 2 minutes .
Repeat the above step one time ( 2nd 70 % wash ) .
Remove as much ethanol as possible without disturbing the pellet Leave tube open on bench ~ 15 minutes .
Dissolve in TE buffer ( ~ pH 7.6 ) .
