Checking DNA Concentration with Agarose Gel
Prepare 0.7 % agarose .
Post - stain with SYBR Gold .
While the gel is cooling , prepare samples : 1µl of 10x loading buffer per 10µl of sample .
Prepare the appropriate dilutions of the appropriate marker ( commonly 10ng of HINDIII [ lambda ] DNA ) .
Add running buffer to cover gel .
Gently rock the comb before pulling it out .
Run at 50V about 2 - 4 hours to get clean band separations .
Post - stain the gel in SYBR Gold ‘bath’ ( 20ul SYBR : 200ml TAE for 30 minutes ) .
Compare gel quantification to that obtained using Nanodrop to get a feeling for how ‘clean’ the DNA prep is .
