DIY Spin Column DNA Extraction
Spin 500ul of bacteria broth culture at 10000g for 10 minutes .
Pour off supernatant .
Add 200ul Lysis buffer and 20ul Proteinase K .
Vortex for 15 seconds and spin down .
Incubate at 56 C for 1 hour .
Add 200ul Binding Buffer and 200ul Ethanol to each sample .
Vortex for 15 seconds and spin down .
Transfer all 600ul of sample to a spin column and collection tube .
Spin on high speed for 1 minute .
Dispose of flow through .
Add 500ul Wash Buffer .
Spin on high speed for 1 minute .
Dispose of flow through .
Add another 500ul Wash Buffer .
Spin on high speed for 1 minute .
Place the spin column in a new collection tube .
Spin on high for 2 minutes .
Place the spin column in a clean tube .
Add 100ul TE buffer .
Incubate at RT for 2 minutes .
Spin on high speed for 1 minute .
Add another 100ul TE buffer .
Incubate at RT for 1 minute .
Spin on high speed for 1 minute .
Discard spin column .
Extracted DNA is in tube .
