T - maze phototaxis with LEDs
Clip wings of half of the flies .
Let flies recover for 1 day ( at 25°C , faster recovery ) .
Put the flies in the T - maze ( entrance tube ) and let them adapt themselves to the machine and the darkness for 10 minutes .
Move the flies into the elevator ( the mobile part of the T - maze ) , and let them stay there for around 1 minute .
Move the flies to the bottom of T - maze ( taking down the elevator ) .
Now they will have to make a decision between two different tubes : one is completely dark , and the other one has a LED in the bottom / is entirely transparent .
We are going to use different LEDs : White LEDs , Yellow LEDs , Green LEDs .
Wait for 30 seconds for the flies to make their decision , and lock them in the tubes that they have chosen ( taking up the elevator ) .
Anesthetize the flies with CO2 and count them under the magnifying glass .
