Western Blot Sample Prep - Whole Drosophila Embryos
Collect 100 embryos and place into eppendorf tube ( 1.5ml ) containing 50ul of embryo wash .
Add 3 drops of bleach and let sit for 3 mins .
Raise volume to 1.5ml using dH2O .
Spin down for 1min .
Remove solution .
Add 1.5ml of dH2O .
Spin down 1min .
Remove solution .
Add 1.5ml of dH2O .
Spin down 1 min .
Remove solution with micropipette .
Add 50ul of 1X PBS .
Add 50ul of 2X SDS SB Homogenize with pestle and 27ga needle .
Boil for 5 mins at 98 degrees C .
Store at - 20 degrees C
