Chlorophyll Extraction in Cyanobacteria
Take 1 ml sample of your cyanobacteria culture and spin it down at 14,000 rpm for 5 min .
Discard 0.9 ml of the supernatant .
Resuspend the pellet in the remaining 100 µl .
Add 0.9 ml of 100 % methanol to the sample and mix thoroughly by vortexing .
Incubate the samples in the dark for 30 min at 4 °C in the fridge .
Spin down samples again at 14,000 rpm for 5 min .
Transfer supernatant into a cuvette and measure the extinction at 665 nm .
Use 90 % methanol as the reference solution .
