Tube - O - Dialyzer ( No Loss Dialyzer for Small Samples )
Tube‐O‐DIALYZER™ are supplied in a preservative to maintain quality .
Prior to use discard the preservative from the tube and place the dialysis cap upside down in a beaker or other suitable container .
Pipette your sample directly into the Tube‐O‐DIALYZER™ tube .
For Tube‐O‐DIALYZER™ Micro use 20‐250µl and for Tube‐O‐DIALYZER™ Medi use 0.2‐2.5ml .
Remove the Tube‐O‐DIALYZER™ dialysis cap from the rinse water / buffer and carefully remove excess liquid with a pipette tip .
Screw the dialysis cap on to the Tube‐O‐DIALYZER™ tube until finger tight .
Invert the Tube‐O‐DIALYZER™ , ensuring the entire sample rests upon the membrane .
Keeping the Tube‐O‐DIALYZER™ in an inverted position , slide the supplied float onto the Tube‐O‐DIALYZER™ tube .
Place the Tube‐O‐DIALYZER™ in the dialysis buffer .
Ensure that the dialysis membrane contacts the dialysis buffer .
If there are large air bubbles trapped underneath the dialysis membrane surface , tilt the tube or squirt buffer to remove the air bubbles .
Dialysis Time : Dialysis time will depend on the nature of sample , MWCO of the Tube‐O‐DIALYZER™ , sample and dialysis buffer volume and concentration .
Higher MWCO will allow faster dialysis .
As a guide , the sample should be dialyzed for 2‐12h .
Dialysis buffer should also be replaced at least once during dialysis .
After dialysis , remove the Tube‐O‐DIALYZER™ from the float and immediately spin the Tube‐O‐DIALYZER™ ( in up‐right position ) for 5‐6 seconds at 500‐1,000xg .
Discard the dialysis cap and replace with the supplied Storage Cap .
Add 1‐2ml DI water or dialysis buffer to rinse .
Keep the Tube‐O‐DIALYZER™ membrane wet until required .
Gently , stir the dialysis buffer .
For efficient and complete dialysis we recommend inverting or gently tapping the Tube‐O‐DIALYZER™ 1‐2 times during dialysis to mix the sample .
